BPA ID Sample Name Project Contact Scientist Institution Species Sample Type RNA Source Extraction Method Growth Protocol Treatment Protocol Experimental Design Notes
BPA ID Sample Name Project Contact Scientist Institution Species Sample Type RNA Source Extraction Method Growth Protocol Treatment Protocol Experimental Design Notes
102.100.100.14324 M42 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) Mock infected 3 dpi biological replicates Mock infected 3 dpi
102.100.100.14325 M43 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) Mock infected 3 dpi biological replicates Mock infected 3 dpi
102.100.100.14326 M48 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) CS3427 infected 3dpi biological replicates CS3427 infected 3dpi
102.100.100.14327 M49 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) CS3427 infected 3dpi biological replicates CS3427 infected 3dpi
102.100.100.14328 M52 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) CS3427 infected 3dpi biological replicates CS3427 infected 3dpi
102.100.100.14329 M114 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) Mock infected 5 dpi biological replicates Mock infected 5 dpi
102.100.100.14330 M115 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) Mock infected 5 dpi biological replicates Mock infected 5 dpi
102.100.100.14331 M116 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) Mock infected 5 dpi biological replicates Mock infected 5 dpi
102.100.100.14332 M119 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) CS3427 infected 5 dpi biological replicates CS3427 infected 5 dpi
102.100.100.14333 M121 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) CS3427 infected 5 dpi biological replicates CS3427 infected 5 dpi
102.100.100.14334 M123 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) CS3427 infected 5 dpi biological replicates CS3427 infected 5 dpi
102.100.100.14335 JA1 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi mock treated wheat roots rep 1
102.100.100.14336 JA2 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi mock treated wheat roots rep 2
102.100.100.14337 JA3 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi mock treated wheat roots rep 3
102.100.100.14338 JA4 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi Rs AG8-1 inoculated wheat roots rep 1
102.100.100.14339 JA5 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi Rs AG8-1 inoculated wheat roots rep 2
102.100.100.14340 JA6 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi Rs AG8-1 inoculated wheat roots rep 3
102.100.100.14343 JA9 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani Small RNA Infected wheat roots Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. Germinated seedlings planted into pre-infected vermiculite 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq 7 dpi mock treated wheat roots rep 3
102.100.100.14350 C.Moffat M1 Pyrenophora in-vitro transcript DrCaroline Moffat Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) Fungus (Pyrenophoratritici-repentis), Plant (wheat) RNA Mycelia (fungus), uninfected and ptr infected leaves (plant) Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser Plant infection assay, whole plant (foliar)spray Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates Vegetative mycelia (isolate Meck4), 7-day-old V8PDA agar plate culture
102.100.100.14351 C.Moffat M2(a) Pyrenophora in-vitro transcript DrCaroline Moffat Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) Fungus (Pyrenophoratritici-repentis), Plant (wheat) RNA Mycelia (fungus), uninfected and ptr infected leaves (plant) Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser Plant infection assay, whole plant (foliar)spray Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates Sporulating mycelia (isolate Meck4),9-day-old V8PDA agar plate culture
102.100.100.14352 C.Moffat D1 Pyrenophora in-vitro transcript DrCaroline Moffat Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) Fungus (Pyrenophoratritici-repentis), Plant (wheat) RNA Mycelia (fungus), uninfected and ptr infected leaves (plant) Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser Plant infection assay, whole plant (foliar)spray Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates Vegetative mycelia (isolate DW5),7-day-old V8PDA agar plate culture
102.100.100.14353 C.Moffat D2(a) Pyrenophora in-vitro transcript DrCaroline Moffat Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) Fungus (Pyrenophoratritici-repentis), Plant (wheat) RNA Mycelia (fungus), uninfected and ptr infected leaves (plant) Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser Plant infection assay, whole plant (foliar)spray Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates Sporulating mycelia (isolate DW5),10-day-old V8PDA agar plate culture
102.100.100.14354 C.Moffat C1 Pyrenophora in-vitro transcript DrCaroline Moffat Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) Fungus (Pyrenophoratritici-repentis), Plant (wheat) RNA Mycelia (fungus), uninfected and ptr infected leaves (plant) Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser Plant infection assay, whole plant (foliar)spray Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates Control leaves (3 DPI),RNA pooled from 3 replicates
102.100.100.14344 JA10 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani Small RNA Infected wheat roots Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. Germinated seedlings planted into pre-infected vermiculite 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq 7 dpi Rs AG8-1 inoculated wheat roots rep 1
102.100.100.14345 JA11 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani Small RNA Infected wheat roots Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. Germinated seedlings planted into pre-infected vermiculite 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq 7 dpi Rs AG8-1 inoculated wheat roots rep 2
102.100.100.14346 JA12 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani Small RNA Infected wheat roots Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. Germinated seedlings planted into pre-infected vermiculite 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq 7 dpi Rs AG8-1 inoculated wheat roots rep 3
102.100.100.14347 JA13 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 1
102.100.100.14348 JA14 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 2
102.100.100.14349 JA15 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 3
102.100.100.14355 C.Moffat P1 Pyrenophora in-vitro transcript DrCaroline Moffat Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) Fungus (Pyrenophoratritici-repentis), Plant (wheat) RNA Mycelia (fungus), uninfected and ptr infected leaves (plant) Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser Plant infection assay, whole plant (foliar)spray Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates Ptr infected leaves (3 DPI),RNA pooled from 3 replicates
102.100.100.14356 C.Moffat C2 Pyrenophora in-vitro transcript DrCaroline Moffat Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) Fungus (Pyrenophoratritici-repentis), Plant (wheat) RNA Mycelia (fungus), uninfected and ptr infected leaves (plant) Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser Plant infection assay, whole plant (foliar)spray Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates Control leaves (4 DPI),RNA pooled from 6 replicates
102.100.100.14357 C.Moffat P2 Pyrenophora in-vitro transcript DrCaroline Moffat Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) Fungus (Pyrenophoratritici-repentis), Plant (wheat) RNA Mycelia (fungus), uninfected and ptr infected leaves (plant) Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser Plant infection assay, whole plant (foliar)spray Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates Ptr infected leaves (4 DPI),RNA pooled from 6 replicates
102.100.100.14358 JA1 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi mock treated wheat roots rep 1
102.100.100.14359 JA2 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi mock treated wheat roots rep 2
102.100.100.14360 JA3 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi mock treated wheat roots rep 3
102.100.100.14361 JA4 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi Rs AG8-1 inoculated wheat roots rep 1
102.100.100.14362 JA5 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi Rs AG8-1 inoculated wheat roots rep 2
102.100.100.14363 JA6 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep 2 dpi Rs AG8-1 inoculated wheat roots rep 3
102.100.100.14364 JA13 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 1
102.100.100.14365 JA14 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 2
102.100.100.14366 JA15 Rhizoctonia infected wheat Jonathan Anderson CSIRO Wheat and Rhizoctonia solani RNA Infected wheat roots Trizol, ribozero ribosomal subtraction kit, strand specific library prep In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 3
102.100.100.14373 Bd21-3 Mock 1 Fusarium pseudograminearum-Brachypodium head necrotrophic interaction Jonathan Powell CSIRO Plant Industry Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 33 seedlings per sample Rneasy Plant Mini Kit
102.100.100.14374 Bd21-3 Mock 2 Fusarium pseudograminearum-Brachypodium head necrotrophic interaction Jonathan Powell CSIRO Plant Industry Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 33 seedlings per sample Rneasy Plant Mini Kit
102.100.100.14375 Bd21-3 Mock 3 Fusarium pseudograminearum-Brachypodium head necrotrophic interaction Jonathan Powell CSIRO Plant Industry Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 33 seedlings per sample Rneasy Plant Mini Kit
102.100.100.14376 Bd21-3 Mock 4 Fusarium pseudograminearum-Brachypodium head necrotrophic interaction Jonathan Powell CSIRO Plant Industry Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 33 seedlings per sample Rneasy Plant Mini Kit
102.100.100.14377 Bd21-3 Fp 1 Fusarium pseudograminearum-Brachypodium head necrotrophic interaction Jonathan Powell CSIRO Plant Industry Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 33 seedlings per sample Rneasy Plant Mini Kit
102.100.100.14378 Bd21-3 Fp 2 Fusarium pseudograminearum-Brachypodium head necrotrophic interaction Jonathan Powell CSIRO Plant Industry Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 33 seedlings per sample Rneasy Plant Mini Kit
102.100.100.14379 Bd21-3 Fp 3 Fusarium pseudograminearum-Brachypodium head necrotrophic interaction Jonathan Powell CSIRO Plant Industry Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 33 seedlings per sample Rneasy Plant Mini Kit
102.100.100.14380 Bd21-3 Fp 4 Fusarium pseudograminearum-Brachypodium head necrotrophic interaction Jonathan Powell CSIRO Plant Industry Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 33 seedlings per sample Rneasy Plant Mini Kit
102.100.100.14381 Chara Mock 1 Fusarium pseudograminearum-wheat head necrotrophic interaction Tim Fitzgerald CSIRO Plant Industry wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 12 seedlings per sample Rneasy Plant Mini Kit Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
102.100.100.14404 4 (Tox1, 24 hpi, I) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14382 Chara Mock 2 Fusarium pseudograminearum-wheat head necrotrophic interaction Tim Fitzgerald CSIRO Plant Industry wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 12 seedlings per sample Rneasy Plant Mini Kit Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
102.100.100.14405 5 (Tox1, 24 hpi, II) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14406 6 (Tox1, 24 hpi, I) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14407 7 (ev, 36 hpi, I) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14408 8 (ev, 36 hpi, II) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14409 9 (ev, 36 hpi, III) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14383 Chara Mock 3 Fusarium pseudograminearum-wheat head necrotrophic interaction Tim Fitzgerald CSIRO Plant Industry wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 12 seedlings per sample Rneasy Plant Mini Kit Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
102.100.100.14418 18 (Tox1, 48 hpi, III) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14384 Chara Mock 4 Fusarium pseudograminearum-wheat head necrotrophic interaction Tim Fitzgerald CSIRO Plant Industry wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 12 seedlings per sample Rneasy Plant Mini Kit Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
102.100.100.14385 Chara Fp 1 Fusarium pseudograminearum-wheat head necrotrophic interaction Tim Fitzgerald CSIRO Plant Industry wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 12 seedlings per sample Rneasy Plant Mini Kit Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
102.100.100.14410 10 (Tox1, 36 hpi, I) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14411 11 (Tox1, 36 hpi, II) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14412 12 (Tox1, 36 hpi, III) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14386 Chara Fp 2 Fusarium pseudograminearum-wheat head necrotrophic interaction Tim Fitzgerald CSIRO Plant Industry wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 12 seedlings per sample Rneasy Plant Mini Kit Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
102.100.100.14389 DG1 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Unger-spores r1
102.100.100.14387 Chara Fp 3 Fusarium pseudograminearum-wheat head necrotrophic interaction Tim Fitzgerald CSIRO Plant Industry wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 12 seedlings per sample Rneasy Plant Mini Kit Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
102.100.100.14413 13 (ev, 48 hpi, I) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14414 14 (ev, 48 hpi, II) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14415 15 (ev, 48 hpi, III) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14416 16 (Tox1, 48 hpi, I) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14417 17 (Tox1, 48 hpi, II) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14388 Chara Fp 4 Fusarium pseudograminearum-wheat head necrotrophic interaction Tim Fitzgerald CSIRO Plant Industry wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum RNA ~ 1.5 cm sections from base of 12 seedlings per sample Rneasy Plant Mini Kit Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
102.100.100.14403 3 (ev, 24 hpi, III) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14390 DG2 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Unger-spores r2
102.100.100.14391 DG3 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Unger-spores r3
102.100.100.14392 DG4 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Infected tissue 0 hai r1
102.100.100.14393 DG5 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Infected tissue 0 hai r2
102.100.100.14394 DG6 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Infected tissue 0 hai r3
102.100.100.14395 DG7 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Infected tissue6 dai r1
102.100.100.14323 M41 Fusarium infected and vegetative tissue - small RNA Donald Gardiner CSIRO Plant Industry Wheat (Fusarium) Small RNA Shoots Trizol Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) Mock infected 3 dpi biological replicates Mock infected 3 dpi
102.100.100.14396 DG8 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Infected tissue6 dai r2
102.100.100.14397 DG9 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Infected tissue6 dai r3
102.100.100.14398 DG10 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Infected tissue9dai
102.100.100.14399 DG11 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Wheat infiltrated with buffer
102.100.100.14400 DG12 Puccinia striiformis infected tissue Diana Garnica and John Rathjen The Australian National University Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) RNA From pst, ungerminated spores, from ta healthy and infected leaves RNAeasy Plant kit QIAGEN Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. Wheat infiltrated with spores extract
102.100.100.14401 1 (ev, 24 hpi, I) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi RIN measured at Ramaciotti
102.100.100.14402 2 (ev, 24 hpi, II) Tox 1 infected wheat Britta Winterberg Australian National University, Research School of Biology Wheat RNA Leaves Kit (Sigma Plant RNeasy Total RNA) The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
102.100.100.14341 JA7 Rhizoctonia infected and vegetative tissue - small RNA Jonathan Anderson CSIRO Wheat and Rhizoctonia solani Small RNA Infected wheat roots Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. Germinated seedlings planted into pre-infected vermiculite 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq 7 dpi mock treated wheat roots rep 2