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| BPA ID | Sample Name | Project | Contact Scientist | Institution | Species | Sample Type | RNA Source | Extraction Method | Growth Protocol | Treatment Protocol | Experimental Design | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BPA ID | Sample Name | Project | Contact Scientist | Institution | Species | Sample Type | RNA Source | Extraction Method | Growth Protocol | Treatment Protocol | Experimental Design | Notes |
| BPAUniqueID object (102.100.100.14324) | M42 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | Mock infected 3 dpi | biological replicates | Mock infected 3 dpi |
| BPAUniqueID object (102.100.100.14325) | M43 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | Mock infected 3 dpi | biological replicates | Mock infected 3 dpi |
| BPAUniqueID object (102.100.100.14326) | M48 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | CS3427 infected 3dpi | biological replicates | CS3427 infected 3dpi |
| BPAUniqueID object (102.100.100.14327) | M49 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | CS3427 infected 3dpi | biological replicates | CS3427 infected 3dpi |
| BPAUniqueID object (102.100.100.14328) | M52 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | CS3427 infected 3dpi | biological replicates | CS3427 infected 3dpi |
| BPAUniqueID object (102.100.100.14329) | M114 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | Mock infected 5 dpi | biological replicates | Mock infected 5 dpi |
| BPAUniqueID object (102.100.100.14330) | M115 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | Mock infected 5 dpi | biological replicates | Mock infected 5 dpi |
| BPAUniqueID object (102.100.100.14331) | M116 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | Mock infected 5 dpi | biological replicates | Mock infected 5 dpi |
| BPAUniqueID object (102.100.100.14332) | M119 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | CS3427 infected 5 dpi | biological replicates | CS3427 infected 5 dpi |
| BPAUniqueID object (102.100.100.14333) | M121 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | CS3427 infected 5 dpi | biological replicates | CS3427 infected 5 dpi |
| BPAUniqueID object (102.100.100.14334) | M123 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | CS3427 infected 5 dpi | biological replicates | CS3427 infected 5 dpi |
| BPAUniqueID object (102.100.100.14335) | JA1 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi mock treated wheat roots rep 1 | |||
| BPAUniqueID object (102.100.100.14336) | JA2 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi mock treated wheat roots rep 2 | |||
| BPAUniqueID object (102.100.100.14337) | JA3 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi mock treated wheat roots rep 3 | |||
| BPAUniqueID object (102.100.100.14338) | JA4 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi Rs AG8-1 inoculated wheat roots rep 1 | |||
| BPAUniqueID object (102.100.100.14339) | JA5 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi Rs AG8-1 inoculated wheat roots rep 2 | |||
| BPAUniqueID object (102.100.100.14340) | JA6 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi Rs AG8-1 inoculated wheat roots rep 3 | |||
| BPAUniqueID object (102.100.100.14343) | JA9 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | Small RNA | Infected wheat roots | Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. | Germinated seedlings planted into pre-infected vermiculite | 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq | 7 dpi mock treated wheat roots rep 3 | |
| BPAUniqueID object (102.100.100.14350) | C.Moffat M1 | Pyrenophora in-vitro transcript | dmoffat | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) | Fungus (Pyrenophoratritici-repentis), Plant (wheat) | RNA | Mycelia (fungus), uninfected and ptr infected leaves (plant) | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser | Plant infection assay, whole plant (foliar)spray | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates | Vegetative mycelia (isolate Meck4), 7-day-old V8PDA agar plate culture |
| BPAUniqueID object (102.100.100.14351) | C.Moffat M2(a) | Pyrenophora in-vitro transcript | dmoffat | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) | Fungus (Pyrenophoratritici-repentis), Plant (wheat) | RNA | Mycelia (fungus), uninfected and ptr infected leaves (plant) | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser | Plant infection assay, whole plant (foliar)spray | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates | Sporulating mycelia (isolate Meck4),9-day-old V8PDA agar plate culture |
| BPAUniqueID object (102.100.100.14352) | C.Moffat D1 | Pyrenophora in-vitro transcript | dmoffat | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) | Fungus (Pyrenophoratritici-repentis), Plant (wheat) | RNA | Mycelia (fungus), uninfected and ptr infected leaves (plant) | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser | Plant infection assay, whole plant (foliar)spray | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates | Vegetative mycelia (isolate DW5),7-day-old V8PDA agar plate culture |
| BPAUniqueID object (102.100.100.14353) | C.Moffat D2(a) | Pyrenophora in-vitro transcript | dmoffat | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) | Fungus (Pyrenophoratritici-repentis), Plant (wheat) | RNA | Mycelia (fungus), uninfected and ptr infected leaves (plant) | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser | Plant infection assay, whole plant (foliar)spray | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates | Sporulating mycelia (isolate DW5),10-day-old V8PDA agar plate culture |
| BPAUniqueID object (102.100.100.14354) | C.Moffat C1 | Pyrenophora in-vitro transcript | dmoffat | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) | Fungus (Pyrenophoratritici-repentis), Plant (wheat) | RNA | Mycelia (fungus), uninfected and ptr infected leaves (plant) | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser | Plant infection assay, whole plant (foliar)spray | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates | Control leaves (3 DPI),RNA pooled from 3 replicates |
| BPAUniqueID object (102.100.100.14344) | JA10 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | Small RNA | Infected wheat roots | Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. | Germinated seedlings planted into pre-infected vermiculite | 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq | 7 dpi Rs AG8-1 inoculated wheat roots rep 1 | |
| BPAUniqueID object (102.100.100.14345) | JA11 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | Small RNA | Infected wheat roots | Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. | Germinated seedlings planted into pre-infected vermiculite | 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq | 7 dpi Rs AG8-1 inoculated wheat roots rep 2 | |
| BPAUniqueID object (102.100.100.14346) | JA12 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | Small RNA | Infected wheat roots | Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. | Germinated seedlings planted into pre-infected vermiculite | 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq | 7 dpi Rs AG8-1 inoculated wheat roots rep 3 | |
| BPAUniqueID object (102.100.100.14347) | JA13 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 1 | |||
| BPAUniqueID object (102.100.100.14348) | JA14 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 2 | |||
| BPAUniqueID object (102.100.100.14349) | JA15 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 3 | |||
| BPAUniqueID object (102.100.100.14355) | C.Moffat P1 | Pyrenophora in-vitro transcript | dmoffat | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) | Fungus (Pyrenophoratritici-repentis), Plant (wheat) | RNA | Mycelia (fungus), uninfected and ptr infected leaves (plant) | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser | Plant infection assay, whole plant (foliar)spray | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates | Ptr infected leaves (3 DPI),RNA pooled from 3 replicates |
| BPAUniqueID object (102.100.100.14356) | C.Moffat C2 | Pyrenophora in-vitro transcript | dmoffat | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) | Fungus (Pyrenophoratritici-repentis), Plant (wheat) | RNA | Mycelia (fungus), uninfected and ptr infected leaves (plant) | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser | Plant infection assay, whole plant (foliar)spray | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates | Control leaves (4 DPI),RNA pooled from 6 replicates |
| BPAUniqueID object (102.100.100.14357) | C.Moffat P2 | Pyrenophora in-vitro transcript | dmoffat | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) | Fungus (Pyrenophoratritici-repentis), Plant (wheat) | RNA | Mycelia (fungus), uninfected and ptr infected leaves (plant) | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser | Plant infection assay, whole plant (foliar)spray | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates | Ptr infected leaves (4 DPI),RNA pooled from 6 replicates |
| BPAUniqueID object (102.100.100.14358) | JA1 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi mock treated wheat roots rep 1 | |||
| BPAUniqueID object (102.100.100.14359) | JA2 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi mock treated wheat roots rep 2 | |||
| BPAUniqueID object (102.100.100.14360) | JA3 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi mock treated wheat roots rep 3 | |||
| BPAUniqueID object (102.100.100.14361) | JA4 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi Rs AG8-1 inoculated wheat roots rep 1 | |||
| BPAUniqueID object (102.100.100.14362) | JA5 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi Rs AG8-1 inoculated wheat roots rep 2 | |||
| BPAUniqueID object (102.100.100.14363) | JA6 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | 2 dpi Rs AG8-1 inoculated wheat roots rep 3 | |||
| BPAUniqueID object (102.100.100.14364) | JA13 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 1 | |||
| BPAUniqueID object (102.100.100.14365) | JA14 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 2 | |||
| BPAUniqueID object (102.100.100.14366) | JA15 | Rhizoctonia infected wheat | janderson | CSIRO | Wheat and Rhizoctonia solani | RNA | Infected wheat roots | Trizol, ribozero ribosomal subtraction kit, strand specific library prep | In vitro grown Rs AG8-1 (defined minimal medium liquid, 25C shaking) rep 3 | |||
| BPAUniqueID object (102.100.100.14373) | Bd21-3 Mock 1 | Fusarium pseudograminearum-Brachypodium head necrotrophic interaction | jpowell | CSIRO Plant Industry | Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 33 seedlings per sample | Rneasy Plant Mini Kit | ||||
| BPAUniqueID object (102.100.100.14374) | Bd21-3 Mock 2 | Fusarium pseudograminearum-Brachypodium head necrotrophic interaction | jpowell | CSIRO Plant Industry | Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 33 seedlings per sample | Rneasy Plant Mini Kit | ||||
| BPAUniqueID object (102.100.100.14375) | Bd21-3 Mock 3 | Fusarium pseudograminearum-Brachypodium head necrotrophic interaction | jpowell | CSIRO Plant Industry | Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 33 seedlings per sample | Rneasy Plant Mini Kit | ||||
| BPAUniqueID object (102.100.100.14376) | Bd21-3 Mock 4 | Fusarium pseudograminearum-Brachypodium head necrotrophic interaction | jpowell | CSIRO Plant Industry | Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 33 seedlings per sample | Rneasy Plant Mini Kit | ||||
| BPAUniqueID object (102.100.100.14377) | Bd21-3 Fp 1 | Fusarium pseudograminearum-Brachypodium head necrotrophic interaction | jpowell | CSIRO Plant Industry | Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 33 seedlings per sample | Rneasy Plant Mini Kit | ||||
| BPAUniqueID object (102.100.100.14378) | Bd21-3 Fp 2 | Fusarium pseudograminearum-Brachypodium head necrotrophic interaction | jpowell | CSIRO Plant Industry | Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 33 seedlings per sample | Rneasy Plant Mini Kit | ||||
| BPAUniqueID object (102.100.100.14379) | Bd21-3 Fp 3 | Fusarium pseudograminearum-Brachypodium head necrotrophic interaction | jpowell | CSIRO Plant Industry | Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 33 seedlings per sample | Rneasy Plant Mini Kit | ||||
| BPAUniqueID object (102.100.100.14380) | Bd21-3 Fp 4 | Fusarium pseudograminearum-Brachypodium head necrotrophic interaction | jpowell | CSIRO Plant Industry | Brachypodium distachyon ecotype Bd21-3 mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 33 seedlings per sample | Rneasy Plant Mini Kit | ||||
| BPAUniqueID object (102.100.100.14381) | Chara Mock 1 | Fusarium pseudograminearum-wheat head necrotrophic interaction | tfitzgerald | CSIRO Plant Industry | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 12 seedlings per sample | Rneasy Plant Mini Kit | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. | |
| BPAUniqueID object (102.100.100.14404) | 4 (Tox1, 24 hpi, I) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14382) | Chara Mock 2 | Fusarium pseudograminearum-wheat head necrotrophic interaction | tfitzgerald | CSIRO Plant Industry | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 12 seedlings per sample | Rneasy Plant Mini Kit | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. | |
| BPAUniqueID object (102.100.100.14405) | 5 (Tox1, 24 hpi, II) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14406) | 6 (Tox1, 24 hpi, I) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14407) | 7 (ev, 36 hpi, I) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14408) | 8 (ev, 36 hpi, II) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14409) | 9 (ev, 36 hpi, III) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14383) | Chara Mock 3 | Fusarium pseudograminearum-wheat head necrotrophic interaction | tfitzgerald | CSIRO Plant Industry | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 12 seedlings per sample | Rneasy Plant Mini Kit | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. | |
| BPAUniqueID object (102.100.100.14418) | 18 (Tox1, 48 hpi, III) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14384) | Chara Mock 4 | Fusarium pseudograminearum-wheat head necrotrophic interaction | tfitzgerald | CSIRO Plant Industry | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 12 seedlings per sample | Rneasy Plant Mini Kit | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. | |
| BPAUniqueID object (102.100.100.14385) | Chara Fp 1 | Fusarium pseudograminearum-wheat head necrotrophic interaction | tfitzgerald | CSIRO Plant Industry | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 12 seedlings per sample | Rneasy Plant Mini Kit | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. | |
| BPAUniqueID object (102.100.100.14410) | 10 (Tox1, 36 hpi, I) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14411) | 11 (Tox1, 36 hpi, II) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14412) | 12 (Tox1, 36 hpi, III) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14386) | Chara Fp 2 | Fusarium pseudograminearum-wheat head necrotrophic interaction | tfitzgerald | CSIRO Plant Industry | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 12 seedlings per sample | Rneasy Plant Mini Kit | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. | |
| BPAUniqueID object (102.100.100.14389) | DG1 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Unger-spores r1 |
| BPAUniqueID object (102.100.100.14387) | Chara Fp 3 | Fusarium pseudograminearum-wheat head necrotrophic interaction | tfitzgerald | CSIRO Plant Industry | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 12 seedlings per sample | Rneasy Plant Mini Kit | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. | |
| BPAUniqueID object (102.100.100.14413) | 13 (ev, 48 hpi, I) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14414) | 14 (ev, 48 hpi, II) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14415) | 15 (ev, 48 hpi, III) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14416) | 16 (Tox1, 48 hpi, I) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14417) | 17 (Tox1, 48 hpi, II) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14388) | Chara Fp 4 | Fusarium pseudograminearum-wheat head necrotrophic interaction | tfitzgerald | CSIRO Plant Industry | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum | RNA | ~ 1.5 cm sections from base of 12 seedlings per sample | Rneasy Plant Mini Kit | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. | |
| BPAUniqueID object (102.100.100.14403) | 3 (ev, 24 hpi, III) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14390) | DG2 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Unger-spores r2 |
| BPAUniqueID object (102.100.100.14391) | DG3 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Unger-spores r3 |
| BPAUniqueID object (102.100.100.14392) | DG4 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Infected tissue 0 hai r1 |
| BPAUniqueID object (102.100.100.14393) | DG5 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Infected tissue 0 hai r2 |
| BPAUniqueID object (102.100.100.14394) | DG6 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Infected tissue 0 hai r3 |
| BPAUniqueID object (102.100.100.14395) | DG7 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Infected tissue6 dai r1 |
| BPAUniqueID object (102.100.100.14323) | M41 | Fusarium infected and vegetative tissue - small RNA | dgardiner | CSIRO Plant Industry | Wheat (Fusarium) | Small RNA | Shoots | Trizol | Wheat cv Kennedy germinated for two days in petri dishes prior to inoculation with either water or Fusarium in an in vitro assay (paper towel) | Mock infected 3 dpi | biological replicates | Mock infected 3 dpi |
| BPAUniqueID object (102.100.100.14396) | DG8 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Infected tissue6 dai r2 |
| BPAUniqueID object (102.100.100.14397) | DG9 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Infected tissue6 dai r3 |
| BPAUniqueID object (102.100.100.14398) | DG10 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Infected tissue9dai |
| BPAUniqueID object (102.100.100.14399) | DG11 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Wheat infiltrated with buffer |
| BPAUniqueID object (102.100.100.14400) | DG12 | Puccinia striiformis infected tissue | diana garnica and john rathjen | The Australian National University | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) | RNA | From pst, ungerminated spores, from ta healthy and infected leaves | RNAeasy Plant kit QIAGEN | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. | Wheat infiltrated with spores extract |
| BPAUniqueID object (102.100.100.14401) | 1 (ev, 24 hpi, I) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | RIN measured at Ramaciotti | |
| BPAUniqueID object (102.100.100.14402) | 2 (ev, 24 hpi, II) | Tox 1 infected wheat | bwinterberg | Australian National University, Research School of Biology | Wheat | RNA | Leaves | Kit (Sigma Plant RNeasy Total RNA) | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi | ||
| BPAUniqueID object (102.100.100.14341) | JA7 | Rhizoctonia infected and vegetative tissue - small RNA | janderson | CSIRO | Wheat and Rhizoctonia solani | Small RNA | Infected wheat roots | Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. | Germinated seedlings planted into pre-infected vermiculite | 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq | 7 dpi mock treated wheat roots rep 2 |