Wheat Pathogens Transcript Sample Information

Project Pyrenophora in-vitro transcript
Sample Name C.Moffat P1
Contact Scientist DrCaroline Moffat
Contact Scientist email Caroline.Moffat@curtin.edu.au
Institution Australian Centre for Necrotrophic Fungal Pathogens (ACNFP)
Species Fungus (Pyrenophoratritici-repentis), Plant (wheat)
Sample Type RNA
RNA Source Mycelia (fungus), uninfected and ptr infected leaves (plant)
Extraction Method Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation
Growth Protocol Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser
Treatment Protocol Plant infection assay, whole plant (foliar)spray
Experimental Design Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates
Note Ptr infected leaves (3 DPI),RNA pooled from 3 replicates
2 Related Sequence files available for BPA Sample ID
Filename MD5 Run Description
CM_P1_TAGCTT_L005_R1_001.fastq.gz 76d8f25217bcc5a9e0d0df84a926714f Run 138 for C.Moffat P1
CM_P1_TAGCTT_L005_R2_001.fastq.gz d269657fe8589825977605654e112105 Run 138 for C.Moffat P1
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