Wheat Pathogens Transcript Sample BPAUniqueID object ( Information

Project Tox 1 infected wheat
Sample Name 13 (ev, 48 hpi, I)
Contact Scientist bwinterberg
Contact Scientist email Britta.winterberg@anu.edu.au
Institution Australian National University, Research School of Biology
Species Wheat
Sample Type RNA
RNA Source Leaves
Extraction Method Kit (Sigma Plant RNeasy Total RNA)
Growth Protocol The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA
Treatment Protocol
Experimental Design 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (
Filename MD5 Run Description
BW13_AGTCAA_L001_R1_001.fastq.gz 0dd857680300f81b9b513a8bf15e82c1 WheatPathogenTranscriptRun object (83)
BW13_AGTCAA_L001_R2_001.fastq.gz 21daf4d86a03a42b77c639f801523aa1 WheatPathogenTranscriptRun object (83)
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