Wheat Pathogens Transcript Sample BPAUniqueID object (102.100.100.14401) Information
| Project | Tox 1 infected wheat |
| Sample Name | 1 (ev, 24 hpi, I) |
| Contact Scientist | bwinterberg |
| Contact Scientist email | Britta.winterberg@anu.edu.au |
| Institution | Australian National University, Research School of Biology |
| Species | Wheat |
| Sample Type | RNA |
| RNA Source | Leaves |
| Extraction Method | Kit (Sigma Plant RNeasy Total RNA) |
| Growth Protocol | The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA |
| Treatment Protocol | |
| Experimental Design | 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi |
| Note | RIN measured at Ramaciotti |
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (102.100.100.14401)
| Filename | MD5 | Run Description |
|---|---|---|
| BW1_CGATGT_L001_R2_001.fastq.gz | ff95a14c35e7089f8fc4697699e03d27 | WheatPathogenTranscriptRun object (71) |
| BW1_CGATGT_L001_R1_001.fastq.gz | a11497f40b0a0d857db045bf5940f032 | WheatPathogenTranscriptRun object (71) |
You can use the URL list below with tools like
wget or curl to fetch only the listed sequence files from the archive.
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/BW1_CGATGT_L001_R2_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/BW1_CGATGT_L001_R1_001.fastq.gz