|Project||Tox 1 infected wheat|
|Sample Name||12 (Tox1, 36 hpi, III)|
|Contact Scientist email||Britta.firstname.lastname@example.org|
|Institution||Australian National University, Research School of Biology|
|Extraction Method||Kit (Sigma Plant RNeasy Total RNA)|
|Growth Protocol||The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA|
|Experimental Design||2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi|
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