Wheat Pathogens Transcript Sample BPAUniqueID object ( Information

Project Rhizoctonia infected and vegetative tissue - small RNA
Sample Name JA7
Contact Scientist janderson
Contact Scientist email
Institution CSIRO
Species Wheat and Rhizoctonia solani
Sample Type Small RNA
RNA Source Infected wheat roots
Extraction Method
Growth Protocol Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2.
Treatment Protocol Germinated seedlings planted into pre-infected vermiculite
Experimental Design 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq
Note 7 dpi mock treated wheat roots rep 2
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (
Filename MD5 Run Description
JA_R7_GTGAAA_L008_R1_001.fastq.gz d7eebe57fcd31b5d055335369c56d5e7 WheatPathogenTranscriptRun object (18)
JA_R7_GTGAAA_L002_R1_001.fastq.gz 6123a30ae8e3660b70828a1e6ac72f44 WheatPathogenTranscriptRun object (18)
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