Wheat Pathogens Transcript Sample Information

Project Fusarium pseudograminearum-wheat head necrotrophic interaction
Sample Name Chara Mock 1
Contact Scientist Tim Fitzgerald
Contact Scientist email timothy.fitzgerald@csiro.au
Institution CSIRO Plant Industry
Species wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum
Sample Type RNA
RNA Source ~ 1.5 cm sections from base of 12 seedlings per sample
Extraction Method Rneasy Plant Mini Kit
Growth Protocol Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature.
Treatment Protocol For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added.
Experimental Design Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type.
2 Related Sequence files available for BPA Sample ID
Filename MD5 Run Description
TF_Chara_Mock1_CGATGT_L007_R2_001.fastq.gz 5bda050b1e37be1774ca3163b6777c74 Run 151 for Chara Mock 1
TF_Chara_Mock1_CGATGT_L007_R1_001.fastq.gz 49c6f2385272853de1f4e1f2971df7d8 Run 151 for Chara Mock 1
You can use the URL list below with tools like wget or curl to fetch only the listed sequence files from the archive.