Wheat Pathogens Transcript Sample 102.100.100.14343 Information

Project Rhizoctonia infected and vegetative tissue - small RNA
Sample Name JA9
Contact Scientist Jonathan Anderson
Contact Scientist email
Institution CSIRO
Species Wheat and Rhizoctonia solani
Sample Type Small RNA
RNA Source Infected wheat roots
Extraction Method
Growth Protocol Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2.
Treatment Protocol Germinated seedlings planted into pre-infected vermiculite
Experimental Design 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq
Note 7 dpi mock treated wheat roots rep 3
2 Related Sequence files available for BPA Sample ID 102.100.100.14343
Filename MD5 Run Description
JA_R9_GTTTCG_L002_R1_001.fastq.gz da2563b3377bcdabe0b228203268f9f1 Run 119 for JA9
JA_R9_GTTTCG_L008_R1_001.fastq.gz 0001416c395b35af969d7c8e1a496449 Run 119 for JA9
You can use the URL list below with tools like wget or curl to fetch only the listed sequence files from the archive.

https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/JA_R9_GTTTCG_L002_R1_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/JA_R9_GTTTCG_L008_R1_001.fastq.gz