Project | Pyrenophora in-vitro transcript |
Sample Name | C.Moffat C1 |
Contact Scientist | dmoffat |
Contact Scientist email | Caroline.Moffat@curtin.edu.au |
Institution | Australian Centre for Necrotrophic Fungal Pathogens (ACNFP) |
Species | Fungus (Pyrenophoratritici-repentis), Plant (wheat) |
Sample Type | RNA |
RNA Source | Mycelia (fungus), uninfected and ptr infected leaves (plant) |
Extraction Method | Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation |
Growth Protocol | Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser |
Treatment Protocol | Plant infection assay, whole plant (foliar)spray |
Experimental Design | Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates |
Note | Control leaves (3 DPI),RNA pooled from 3 replicates |
Filename | MD5 | Run Description |
---|---|---|
CM_C1_CGATGT_L005_R2_001.fastq.gz | d0c6b5d48d2f885cdb3d48ee629177ab | WheatPathogenTranscriptRun object (30) |
CM_C1_CGATGT_L005_R1_001.fastq.gz | cd53b8736ce0bd5204b799e36343bbce | WheatPathogenTranscriptRun object (30) |
wget
or curl
to fetch only the listed sequence files from the archive.
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/CM_C1_CGATGT_L005_R2_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/CM_C1_CGATGT_L005_R1_001.fastq.gz