Project | Fusarium pseudograminearum-wheat head necrotrophic interaction |
Sample Name | Chara Fp 3 |
Contact Scientist | tfitzgerald |
Contact Scientist email | timothy.fitzgerald@csiro.au |
Institution | CSIRO Plant Industry |
Species | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum |
Sample Type | RNA |
RNA Source | ~ 1.5 cm sections from base of 12 seedlings per sample |
Extraction Method | Rneasy Plant Mini Kit |
Growth Protocol | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. |
Treatment Protocol | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. |
Experimental Design | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. |
Note |
Filename | MD5 | Run Description |
---|---|---|
TF_Chara_Fp3_CCGTCC_L007_R1_001.fastq.gz | d035f4d278cdce64e1578537200e0887 | WheatPathogenTranscriptRun object (57) |
TF_Chara_Fp3_CCGTCC_L007_R2_001.fastq.gz | 88a82289225a1f5fc61d3a84b3aaa5d1 | WheatPathogenTranscriptRun object (57) |
wget
or curl
to fetch only the listed sequence files from the archive.
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/TF_Chara_Fp3_CCGTCC_L007_R1_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/TF_Chara_Fp3_CCGTCC_L007_R2_001.fastq.gz