Wheat Pathogens Transcript Sample BPAUniqueID object ( Information

Project Pyrenophora in-vitro transcript
Sample Name C.Moffat D1
Contact Scientist dmoffat
Contact Scientist email Caroline.Moffat@curtin.edu.au
Institution Australian Centre for Necrotrophic Fungal Pathogens (ACNFP)
Species Fungus (Pyrenophoratritici-repentis), Plant (wheat)
Sample Type RNA
RNA Source Mycelia (fungus), uninfected and ptr infected leaves (plant)
Extraction Method Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation
Growth Protocol Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser
Treatment Protocol Plant infection assay, whole plant (foliar)spray
Experimental Design Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates
Note Vegetative mycelia (isolate DW5),7-day-old V8PDA agar plate culture
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (
Filename MD5 Run Description
CM_D1_CAGATC_L005_R2_001.fastq.gz 18735436d2cbe53327f9281265db8a36 WheatPathogenTranscriptRun object (28)
CM_D1_CAGATC_L005_R1_001.fastq.gz 066a216576fc87f75476bcd5fa50cb9b WheatPathogenTranscriptRun object (28)
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