Wheat Pathogens Transcript Sample 102.100.100.14417 Information

Project Tox 1 infected wheat
Sample Name 17 (Tox1, 48 hpi, II)
Contact Scientist Britta Winterberg
Contact Scientist email Britta.winterberg@anu.edu.au
Institution Australian National University, Research School of Biology
Species Wheat
Sample Type RNA
RNA Source Leaves
Extraction Method Kit (Sigma Plant RNeasy Total RNA)
Growth Protocol The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA
Treatment Protocol
Experimental Design 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
Note
2 Related Sequence files available for BPA Sample ID 102.100.100.14417
Filename MD5 Run Description
BW17_GTGAAA_L002_R1_001.fastq.gz b013b22aad92f9bca59333b8402c5345 Run 169 for 17 (Tox1, 48 hpi, II)
BW17_GTGAAA_L002_R2_001.fastq.gz 772fb98c6a4dbcd29e37e41348a8f615 Run 169 for 17 (Tox1, 48 hpi, II)
You can use the URL list below with tools like wget or curl to fetch only the listed sequence files from the archive.

https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/BW17_GTGAAA_L002_R1_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/BW17_GTGAAA_L002_R2_001.fastq.gz