Wheat Pathogens Transcript Sample BPAUniqueID object (102.100.100.14414) Information

Project Tox 1 infected wheat
Sample Name 14 (ev, 48 hpi, II)
Contact Scientist bwinterberg
Contact Scientist email Britta.winterberg@anu.edu.au
Institution Australian National University, Research School of Biology
Species Wheat
Sample Type RNA
RNA Source Leaves
Extraction Method Kit (Sigma Plant RNeasy Total RNA)
Growth Protocol The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA
Treatment Protocol
Experimental Design 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
Note
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (102.100.100.14414)
Filename MD5 Run Description
BW14_AGTTCC_L002_R2_001.fastq.gz 119d3fd566fcbf32ff71a519ee2f44e2 WheatPathogenTranscriptRun object (84)
BW14_AGTTCC_L002_R1_001.fastq.gz e6550d2eebdb7e5aaa05f24f50648ec9 WheatPathogenTranscriptRun object (84)
You can use the URL list below with tools like wget or curl to fetch only the listed sequence files from the archive.

https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/BW14_AGTTCC_L002_R2_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/BW14_AGTTCC_L002_R1_001.fastq.gz