Wheat Pathogens Transcript Sample Information

Project Tox 1 infected wheat
Sample Name 3 (ev, 24 hpi, III)
Contact Scientist Britta Winterberg
Contact Scientist email Britta.winterberg@anu.edu.au
Institution Australian National University, Research School of Biology
Species Wheat
Sample Type RNA
RNA Source Leaves
Extraction Method Kit (Sigma Plant RNeasy Total RNA)
Growth Protocol The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA
Treatment Protocol
Experimental Design 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
2 Related Sequence files available for BPA Sample ID
Filename MD5 Run Description
BW3_TTAGGC_L003_R1_001.fastq.gz 4eeee76ede1426e7627aa94f63827034 Run 169 for 3 (ev, 24 hpi, III)
BW3_TTAGGC_L003_R2_001.fastq.gz b58e63f7fdf6eee4b91d181563303744 Run 169 for 3 (ev, 24 hpi, III)
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