Wheat Pathogens Transcript Sample 102.100.100.14415 Information

Project Tox 1 infected wheat
Sample Name 15 (ev, 48 hpi, III)
Contact Scientist Britta Winterberg
Contact Scientist email Britta.winterberg@anu.edu.au
Institution Australian National University, Research School of Biology
Species Wheat
Sample Type RNA
RNA Source Leaves
Extraction Method Kit (Sigma Plant RNeasy Total RNA)
Growth Protocol The fungal effector Tox1 was expressed in Pichia and purified from liquid cultures, empty vector-transformed Pichia served as control, 12-day old seedling leaves were infiltrated with purified fungal effector Tox1 or empty vector control, Samples were collected 24, 36 and 48 hours post infiltration in biological triplicates, for each sample three leave samples were collected covering the infiltration site, samples were immediately frozen in liquid N2, stored at -80C until extraction of RNA
Treatment Protocol
Experimental Design 2 different treatments: Tox1 and empty vector (ev) control, 3 biological replicates (I, II, III), timepoints: 24, 36 and 48 hpi
Note
2 Related Sequence files available for BPA Sample ID 102.100.100.14415
Filename MD5 Run Description
BW15_TAGCTT_L003_R1_001.fastq.gz 84639fd43ada63fdb5a846e84fd28982 Run 169 for 15 (ev, 48 hpi, III)
BW15_TAGCTT_L003_R2_001.fastq.gz 64d83b6373923582182effd8df0fec89 Run 169 for 15 (ev, 48 hpi, III)
You can use the URL list below with tools like wget or curl to fetch only the listed sequence files from the archive.

https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/BW15_TAGCTT_L003_R1_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/BW15_TAGCTT_L003_R2_001.fastq.gz