Wheat Pathogens Transcript Sample BPAUniqueID object (102.100.100.14346) Information
| Project | Rhizoctonia infected and vegetative tissue - small RNA |
| Sample Name | JA12 |
| Contact Scientist | janderson |
| Contact Scientist email | |
| Institution | CSIRO |
| Species | Wheat and Rhizoctonia solani |
| Sample Type | Small RNA |
| RNA Source | Infected wheat roots |
| Extraction Method | |
| Growth Protocol | Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2. |
| Treatment Protocol | Germinated seedlings planted into pre-infected vermiculite |
| Experimental Design | 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq |
| Note | 7 dpi Rs AG8-1 inoculated wheat roots rep 3 |
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (102.100.100.14346)
| Filename | MD5 | Run Description |
|---|---|---|
| JA_R12_GGTAGC_L002_R1_001.fastq.gz | 8bca621e88b62123405b7a469cb93529 | WheatPathogenTranscriptRun object (22) |
| JA_R12_GGTAGC_L008_R1_001.fastq.gz | b6e4d3d4c7d71d47bb5dfcbb5037cf28 | WheatPathogenTranscriptRun object (22) |
You can use the URL list below with tools like
wget or curl to fetch only the listed sequence files from the archive.
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/JA_R12_GGTAGC_L002_R1_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/JA_R12_GGTAGC_L008_R1_001.fastq.gz