Wheat Pathogens Transcript Sample BPAUniqueID object ( Information

Project Rhizoctonia infected and vegetative tissue - small RNA
Sample Name JA12
Contact Scientist janderson
Contact Scientist email
Institution CSIRO
Species Wheat and Rhizoctonia solani
Sample Type Small RNA
RNA Source Infected wheat roots
Extraction Method
Growth Protocol Moist vermiculite pre-infected with fungus, germinated seedlings planted to vermiculite, harvest at 2, 4, 7 and 10 days after planting. RNA-seq at 2 and 7 dpi. Additional plants left until 21 dpi to confirm infective conditions. Amount of fungal DNA in-planta also confirmed infection occurring from day 2.
Treatment Protocol Germinated seedlings planted into pre-infected vermiculite
Experimental Design 3 biological replicates for all samples. QPCR confirmation of wheat response and fungal gene expression prior to RNA-seq
Note 7 dpi Rs AG8-1 inoculated wheat roots rep 3
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (
Filename MD5 Run Description
JA_R12_GGTAGC_L002_R1_001.fastq.gz 8bca621e88b62123405b7a469cb93529 WheatPathogenTranscriptRun object (22)
JA_R12_GGTAGC_L008_R1_001.fastq.gz b6e4d3d4c7d71d47bb5dfcbb5037cf28 WheatPathogenTranscriptRun object (22)
You can use the URL list below with tools like wget or curl to fetch only the listed sequence files from the archive.