Wheat Pathogens Transcript Sample BPAUniqueID object (102.100.100.14384) Information
| Project | Fusarium pseudograminearum-wheat head necrotrophic interaction |
| Sample Name | Chara Mock 4 |
| Contact Scientist | tfitzgerald |
| Contact Scientist email | timothy.fitzgerald@csiro.au |
| Institution | CSIRO Plant Industry |
| Species | wheat (Triticum aestivum) cv. Chara mock inoculated/inoculated with Fusarium pseudograminearum |
| Sample Type | RNA |
| RNA Source | ~ 1.5 cm sections from base of 12 seedlings per sample |
| Extraction Method | Rneasy Plant Mini Kit |
| Growth Protocol | Seedlings were inoculated at 3 days post germination and wrapped in paper towel as per Yang et al. 2010 European Journal of Plant Pathology 128; 495-502, then grown for 3 days under constant light in the laboratory. CS3427 fungus was cultured from a V8 agar plug in _ V8 media in flasks at room temperature. |
| Treatment Protocol | For each sample, 3 days post-germination seedlings were placed into Falcon tubes. 3 mls Fusarium pseudograminearum CS3427 spores at a concentration of 5 x 10^5 (in sterile water), or sterile water for mock samples, was added. Tubes were rotated on a tube roller at medium speed setting for 5 minutes. Inoculum was drained and seedlings were placed onto a fresh piece of paper towel to remove excess inoculum. For each sample, 4 seedlings were rolled into each of three paper towel rolls and each roll was placed into an individual Falcon tube and 35 mls sterile water added. |
| Experimental Design | Treatment types: Two treatments, 3 days post inoculation and 3 days post mock inoculation. Biological replication: Tissue from twelve individual seedlings pooled for each sample. Sample number: Four samples per treatment type. |
| Note |
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (102.100.100.14384)
| Filename | MD5 | Run Description |
|---|---|---|
| TF_Chara_Mock4_AGTCAA_L007_R1_001.fastq.gz | fb6353374f2eeff468a89576a1c0fcd6 | WheatPathogenTranscriptRun object (54) |
| TF_Chara_Mock4_AGTCAA_L007_R2_001.fastq.gz | c3b3207ce704ad9a729355a39a416b48 | WheatPathogenTranscriptRun object (54) |
You can use the URL list below with tools like
wget or curl to fetch only the listed sequence files from the archive.
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/TF_Chara_Mock4_AGTCAA_L007_R1_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/TF_Chara_Mock4_AGTCAA_L007_R2_001.fastq.gz