Wheat Pathogens Transcript Sample BPAUniqueID object (102.100.100.14400) Information
| Project | Puccinia striiformis infected tissue |
| Sample Name | DG12 |
| Contact Scientist | diana garnica and john rathjen |
| Contact Scientist email | diana.garnica@anu.edu.au |
| Institution | The Australian National University |
| Species | Pucciniastriiformisf.sptritici (Pst) and Triticumaestivum(Ta) |
| Sample Type | RNA |
| RNA Source | From pst, ungerminated spores, from ta healthy and infected leaves |
| Extraction Method | RNAeasy Plant kit QIAGEN |
| Growth Protocol | Wheat seedling were grown at 21C for two weeks, then infected with fungal spores and maintained in a wet chamber at 9C for 48h. Subsequently plants were moved to a growth chamber at 17C in 16/8 light cycle. Collection of infected tissue was done a different time points during the process mentioned above. Healthy tissue was collected from uninfected plants. Germinated spores were collected ~17 days after infection when the plant tissue is fully covered in spores. |
| Treatment Protocol | Ungerminated spores of the pathogen are mixed with talk and sprayed onto wet wheat seedlings. |
| Experimental Design | Three biological replicates from different time points along the infection were collected and sequenced (0 hai and6 dai). Only one sample 9 dai was collected s in the past we had sequenced two replicates. Also, three replicates of ungerminated spores were sequenced. Finally, one replicate of wheat leave tissue infiltrated with buffer and one replicate of wheat leave tissue infiltrated with spores extract were sequenced. |
| Note | Wheat infiltrated with spores extract |
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (102.100.100.14400)
| Filename | MD5 | Run Description |
|---|---|---|
| DG_12_ATTCCT_L001_R1_001.fastq.gz | e3c37e94cf090050bac28128d4033d6b | WheatPathogenTranscriptRun object (70) |
| DG_12_ATTCCT_L001_R2_001.fastq.gz | dc09fafada814627d76dc18e8b3f1746 | WheatPathogenTranscriptRun object (70) |
You can use the URL list below with tools like
wget or curl to fetch only the listed sequence files from the archive.
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/DG_12_ATTCCT_L001_R1_001.fastq.gz
https://downloads-qcif.bioplatforms.com/bpa/wheat_pathogens_transcript/all/DG_12_ATTCCT_L001_R2_001.fastq.gz