Wheat Pathogens Transcript Sample BPAUniqueID object ( Information

Project Pyrenophora in-vitro transcript
Sample Name C.Moffat M1
Contact Scientist dmoffat
Contact Scientist email Caroline.Moffat@curtin.edu.au
Institution Australian Centre for Necrotrophic Fungal Pathogens (ACNFP)
Species Fungus (Pyrenophoratritici-repentis), Plant (wheat)
Sample Type RNA
RNA Source Mycelia (fungus), uninfected and ptr infected leaves (plant)
Extraction Method Trizol reagent and column purification; Trizol reagent andcolumn purification followed by LiCl re-precipitation
Growth Protocol Fungus Ptr cultures grown on V8PDA agar, Plant Wheat seedlings grown on vermiculite without fertiliser
Treatment Protocol Plant infection assay, whole plant (foliar)spray
Experimental Design Fungal mycelia, vegetative and sporulating mycelia harvested at one time point Plant infection assay fungal infected and uninfected wheat leaves harvested at three and four DPI, RNA pooled from biological replicates
Note Vegetative mycelia (isolate Meck4), 7-day-old V8PDA agar plate culture
2 Related Sequence files available for BPA Sample ID BPAUniqueID object (
Filename MD5 Run Description
CM_M1_ATCACG_L005_R1_001.fastq.gz da9ef04507ea0ef1ce9d88ff4c977470 WheatPathogenTranscriptRun object (26)
CM_M1_ATCACG_L005_R2_001.fastq.gz 4c41c2366c5328229123b41652ba27d6 WheatPathogenTranscriptRun object (26)
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