Ghost bat, Transcriptome, Illumina FASTQ, Testis
Dataset size is: 4.92 GiB
Data and Resources
This data is made available openly under a Creative Commons Attribution license.
Please see the initiative
Data Policy
for attribution information.
Additional Info Show Blank Fields
| Field | Value |
|---|---|
| Resource Permissions | organization_member_after_embargo:date_of_transfer_to_archive:365:tsi-consortium-members |
| Access Control Date | 2022-04-14 |
| Access Control Mode | date |
| Sequence Data Type | illumina-shortread |
| access_rights | exact site coordinates should not be released to public as it is an important conservation site for ghost bats |
| analysis_software | Bcl2fastq2 |
| analysis_software_version | v2.20 |
| ancillary_notes | The captured bat was clearly an older bat with very worn teeth, scarred wing membrane and a large notch missing from his ear. |
| associated_media | photos,videos |
| base_url | https://downloads-qcif.bioplatforms.com/bpa/tsi_staging/illumina-fastq/BPAOPS-1062/20210414_TSI_UNSW_H2KN2DRXY/ |
| ccg_jira_ticket | BPAOPS-1062 |
| certainty | Certain |
| class | Mammalia |
| collection_date | 2020-09-23 |
| collection_method | A ghost bat was captured using a mist net in open woodland in the vicinity of a known roost. Bat was lured into the mist net using a playback protocol designed by Nicola Hanrahan during her PhD research. Bat was removed from net immediately and placed into a calico bag. The ghost bat was sedated by a trained vet to a workable sedation using isoflourane in a purpose-built chamber. Blood samples were taken using cranial vena cava venepuncture. The fully sedated bat was then euthanised using cervical dislocation. Once the bat was confirmed to be deceased, samples were taken from all identifiable organs, with the number of samples taken based on the size of the organ. |
| collector | Nicola Hanrahan (Western Sydney University/Charles Darwin University), Simon Hollamby (Veterinary Surgeon; Territory Wildlife Park), Damian Stanioch (NT Parks & Wildlife), Sam Banks (Charles Darwin University), Brenton von Takach Dukai (Charles Darwin University) |
| collector_sample_id | PC43 |
| common_name | Ghost bat |
| country | Australia |
| data_context | Transcriptome |
| data_custodian | Nicola Hanrahan |
| data_type | Illumina FASTQ |
| dataset_id | 102.100.100/358738 |
| date_of_transfer | 2021-04-14 |
| date_of_transfer_to_archive | 2021-04-15 |
| death_date | 2020-09-23 |
| facility_project_code | Q0005470 |
| facility_sample_id | FAR9073A12 |
| family | Megadermatidae |
| file_name | 355624_TSI_UNSW_H2KN2DRXY_CTGCGAGCCA-TGGCCGGATT |
| flowcell_id | H2KN2DRXY |
| flowcell_type | NovaSeq S1 |
| folder_name | 20210414_TSI_UNSW_H2KN2DRXY |
| genus | Macroderma |
| habitat | Undulating hills, Open Eucalypt woodland, historic gold mining area with multiple mine shafts. |
| insert_size_range | 160.0 |
| institution_name | Charles Darwin University |
| library_construction_protocol | Illumina Stranded mRNA Prep Ligation |
| library_id | 102.100.100/357635 |
| library_index_id | UDP0044-UDP0044A_D06 |
| library_index_seq | CTGCGAGCCA-TGGCCGGATT |
| library_layout | paired end |
| library_location | UNSW |
| library_ng_ul | 23.8 |
| library_pcr_cycles | 9.0 |
| library_pcr_reps | 1.0 |
| library_prep_date | 2021-04-07 |
| library_prepared_by | Caitriona Murray |
| library_selection | PolyA |
| library_source | Transcriptomic |
| library_status | Library sequenced |
| library_strategy | ssRNA-seq |
| library_type | stranded mRNA |
| lifestage | adult |
| location_text | Pine Creek Township, Northern Territroy |
| material_conc_ng_ul | 185.8 |
| material_extracted_by | Elspeth McLennan; Kate Farquharson |
| material_extraction_date | 2020-12-07 |
| material_extraction_method | Qiagen RNeasy Mini Kit with DNAse treatment |
| material_extraction_type | RNA |
| metadata_revision_date | 2023-11-30 |
| metadata_revision_filename | 20231130_Sample_metadata_COMBINED-FOR-PORTAL_NOLATLON.xlsx |
| method_of_determination | Genitals |
| n_libraries_pooled | 27.0 |
| order | Chiroptera |
| phenotypic_sex | male |
| phylum | Chordata |
| prior_genetics | Genome sequencing |
| sample_custodian | Nicola Hanrahan |
| sample_id | 102.100.100/355624 |
| sample_quality | High |
| scientific_name | Macroderma gigas |
| sequencing_facility | UNSW |
| sequencing_model | Illumina NovaSeq 6011 |
| sequencing_platform | Illumina |
| source_population | Pine Creek |
| species | gigas |
| specimen_id | MG_PC43 |
| specimen_id_description | scientific name acronym_researcher assigned sample ID |
| state_or_region | Northern Territory |
| taxon_id | 9411.0 |
| taxonomic_group | Mammal |
| ticket | BPAOPS-1062 |
| tissue_collection | Charles Darwin University_Nicola Hanrahan |
| tissue_number | MG_PC43_testis |
| tissue_preservation | RNAlater - Placed in tube with 1ml RNALater,flash frozen in liquid nitrogen, transported on dry ice and stored in -80 freezer |
| tissue_type | Testis |
| wild_captive | wild_caught |
| work_order | 14009 |