Stemcell Metabolomics 102.100.100/24721 LC/MS
Dataset size is: 74.23 MiB
Data and Resources
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Additional Info Show Blank Fields
| Field | Value |
|---|---|
| Resource Permissions | organization_member |
| Sequence Data Type | metabolomics |
| acquisition_mode | Scan (85-1200 m/z; 0.8 spectra/s) |
| analytical_platform | LC/MS |
| archive_ingestion_date | 2017-03-24 |
| bpa_dataset_id | 102.100.100/52058 |
| cell_type | C11 hiPSCs were genomed edited to knock in a fluorescent protein into the endogenous SOX10 locus. This line was differentiated to neural crest cells. SOX10 positive and negative cells were harvested for analysis |
| contextual_data_submission_date | 2016-01-05 |
| data_generated | 2017-03-24 |
| data_type | mzML and tar.gz files |
| dataset_url | https://downloads-qcif.bioplatforms.com/bpa/stemcell/raw/metabolomic/ma/BPAOPS-84/ |
| date_of_transfer | 2017-03-24 |
| description | Wolvertang LC-MS cell samples-Raw |
| disease_state | WT |
| extract_protocol | As per provided by metabolomics australia |
| facility | MA - Bio21 |
| folder_name | 20170324_MA_BPA_SC_20160630_LCMS |
| instrument_column_type | Agilent 1200 LC system/SeQuant ZIC-p HILIC column (5µm, 150 x 4.6 mm; Millipore) |
| mass_spectrometer | Agilent Q-TOF mass spectrometer 6545 |
| method | Binary gradient made up of solvent A, 20mM ammonium carbonate solution made up in water, and solvent B, 100% acetonitrile using flow rate of 0.3mL/minute. Solvent B gradient was reduced from 80% to 50% between 0.5 and 15.5 minutes, then reduced to 30% between 15.5 and 17.5 minutes. This gradient was further reduced to 5% between 17.5 and 18.5 minutes, kept at 5% until 23 minutes, increased back up to 80% after 23 minutes and kept at 80% until 29 minutes. |
| omics | Metabolomics |
| organism_part | human induced pluripotent stem cells differentiated to neural crest cells in vitro |
| plate_number | 1 |
| replicate_group_id | pos_1 |
| research_group | Ernst Wolvetang |
| sample_description | Medium and Supernatant after extraction |
| sample_fractionation_extraction_solvent | Polar metabolite / Methanol:Water |
| sample_id | 102.100.100/24721 |
| sample_name | 129 |
| sample_submission_date | 2016-01-05 |
| species | Homo sapiens |
| stem_cell_line | PSC - Neural crest |
| stem_cell_state | Pluripotent stem cell state |
| submitter | Anu Balachandran |
| ticket | BPAOPS-84 |
| total_samples | 10 |
| well_number | 1 |