ARP Proteomics SwathMS 51258
Dataset size is: 1.32 GiB
Data and Resources
This data is made available openly under a Creative Commons Attribution license.
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Additional Info Show Blank Fields
| Field | Value |
|---|---|
| Resource Permissions | organization_member |
| Sequence Data Type | proteomics |
| analytical_platform | SWATH-MS |
| contact_researcher | Mark Walker |
| contextual_data_submission_date | 2018-04-05 |
| culture_collection_date | 2008-04-28 |
| culture_collection_id | SP444 |
| data_generated | True |
| data_type | Main dataset |
| facility | APAF |
| gradient_time_per_acn | 600nL/min, 2% to 10% B, 10 min, 10% B to 35% B, 78 min |
| gram_stain | POS |
| growth_condition_growth_phase | Mid log phase |
| growth_condition_media | RPMI_modified |
| growth_condition_od600_reading | 0.4 |
| growth_condition_temperature | 37.0 |
| growth_condition_time_(h) | 1.5 hrs |
| growth_media | RPMI_modified |
| host_age | NA |
| host_description | NA |
| host_disease_outcome | Isolated during invasive disease outbreak |
| host_dob | 2006-02-19 |
| host_location | Queensland, Australia |
| host_sex | M |
| isolation_source | Blood |
| key_virulence_genes | SpeB +ve, no capsule |
| lc_column_type | Eksigent Ultra nanoLC system (Eksigent), Halo C18, 100 mm × 150 µm, 160Å, 2.7 µm |
| mass_spectrometer | 6600 Triple TOF |
| omics | Proteomics |
| peptide_resuspension_protocol | Tip-probe sonicate 2x 30s in solvent intended for injection (e.g 90% MeCN/0.1% TFA) |
| protein_yield_facility | 232.0 |
| protein_yield_total | 464.0 |
| publication_reference | JBC 289: 32303-32315 (2014) |
| replicate | 5 |
| sample_id | 102.100.100/51258 |
| sample_submission_date | 2018-04-05 |
| sample_type | HLB-cleaned dried peptides |
| serovar | M4 |
| strain_description | Isolated during invasive disease outbreak |
| strain_or_isolate | SP444 |
| taxon_or_organism | Streptococcus pyogenes |
| ticket | BPAOPS-696 |
| treatment | 10mM DTT, 20mM IAA, 1:25µg trypsin 37C 24h |