ARP Proteomics SwathMS 51253
Dataset size is: 2.79 GiB
Data and Resources
This data is made available openly under a Creative Commons Attribution license.
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Additional Info Show Blank Fields
| Field | Value |
|---|---|
| Resource Permissions | organization_member |
| Sequence Data Type | proteomics |
| analytical_platform | SWATH-MS |
| contact_researcher | Mark Walker |
| contextual_data_submission_date | 2018-04-05 |
| culture_collection_date | pre-2000 |
| culture_collection_id | SP137 |
| data_generated | True |
| data_type | Main dataset |
| facility | APAF |
| gradient_time_per_acn | 600nL/min, 2% to 10% B, 10 min, 10% B to 35% B, 78 min |
| gram_stain | POS |
| growth_condition_growth_phase | N/A |
| growth_condition_media | Pooled sera |
| growth_condition_od600_reading | N/A |
| growth_condition_temperature | 37.0 |
| growth_condition_time_(h) | 2 hrs exposure to sera |
| growth_media | Pooled sera |
| host_age | NA |
| host_description | NA |
| host_disease_outcome | Toxic shock syndrome and necrotizing fasciitis |
| host_dob | NA |
| host_location | Ontario, Canada |
| isolation_source | Blood |
| key_virulence_genes | SpeB +ve |
| lc_column_type | Eksigent Ultra nanoLC system (Eksigent), Halo C18, 100 mm × 150 µm, 160Å, 2.7 µm |
| mass_spectrometer | 6600 Triple TOF |
| omics | Proteomics |
| peptide_resuspension_protocol | Tip-probe sonicate 2x 30s in solvent intended for injection (e.g 90% MeCN/0.1% TFA) |
| protein_yield_facility | 90.0 |
| protein_yield_total | 180.0 |
| publication_reference | Infect Immun 68: 6362-6369 (2000) |
| replicate | 6 |
| sample_id | 102.100.100/51253 |
| sample_submission_date | 2018-04-05 |
| sample_type | HLB-cleaned dried peptides |
| serovar | M1T1 clone |
| strain_description | Extensively studied, global reference strain. Representative of serotype M1T1 clone. Tn-seq library available |
| strain_or_isolate | 5448 |
| taxon_or_organism | Streptococcus pyogenes |
| ticket | BPAOPS-696 |
| treatment | 10mM DTT, 20mM IAA, 1:25µg trypsin 37C 24h |