ARP Proteomics SwathMS 50174
Dataset size is: 2.21 GiB
Data and Resources
This data is made available openly under a Creative Commons Attribution license.
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Additional Info Show Blank Fields
| Field | Value |
|---|---|
| Resource Permissions | organization_member |
| Sequence Data Type | proteomics |
| additional_notes | 20 ml culture_mutanolysin |
| analytical_platform | SWATH-MS |
| archive_ingestion_date | 2018-07-12 |
| contact_researcher | James Paton/Richard Harvey |
| contextual_data_submission_date | 2017-10-31 |
| culture_collection_date | 1988-06-07 |
| data_generated | True |
| data_type | Main dataset |
| facility | APAF |
| gradient_time_per_acn | 600nL/min, 2% to 10% B, 10 min, 10% B to 35% B, 78 min |
| gram_stain | POS |
| growth_condition_growth_phase | Mid log phase |
| growth_condition_media | RPMI + galactose |
| growth_condition_od600_reading | 0.25 |
| growth_condition_temperature | 37.0 |
| growth_media | RPMI + galactose |
| host_location | Victoria, Australia (Melbourne) |
| host_sex | M |
| isolation_source | Blood |
| key_virulence_genes | Low hemolytic Ply |
| lc_column_type | Eksigent Ultra nanoLC system (Eksigent), Halo C18, 100 mm × 150 µm, 160Å, 2.7 µm |
| mass_spectrometer | 6600 Triple TOF |
| omics | Proteomics |
| protein_yield_facility | 37.0 |
| protein_yield_total | 74.0 |
| publication_reference | Infect. Immun. 79(1):4122-30 (2011) |
| replicate | 6 |
| sample_id | 102.100.100/50174 |
| sample_submission_date | 2017-10-31 |
| sample_type | Dried peptides |
| serovar | 1 |
| strain_description | Highly virulent in animal models (ST3018) |
| strain_or_isolate | 4496 |
| taxon_or_organism | Streptococcus pneumoniae |
| ticket | BPAOPS-627 |
| treatment | 25U mutanolysin 37C 2h, 10mM DTT, 20mM IAA, 1:25 ug trypsin |