Sepsis Genomics Miseq 25788
Dataset size is: 326.94 MiB
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Additional Info Show Blank Fields
| Field | Value |
|---|---|
| Resource Permissions | organization_member |
| Sequence Data Type | illumina-shortread |
| analysis_software_version | 2.6.2.3 |
| analytical_facility | Ramaciotti |
| archive_ingestion_date | 2016-08-18 |
| contact_researcher | James Paton/Claudia Trappetti |
| contextual_data_submission_date | 2016-04-26 |
| culture_collection_date | 1989-07-28 |
| data_generated | True |
| data_type | Main dataset |
| experimental_replicate | 1 |
| experimental_sample_preparation_method | Qiagen kit with 100/G genomic tip |
| facility | RAMACIOTTI |
| gram_stain | POS |
| growth_condition_media | THY broth |
| growth_condition_notes | THY broth (recommend using filtered not autoclaved broth as some of the strains don't seem to grow when media has been overheated). If pelleting, serotype 3 forms large loose pellets compared to other pneumococci due to slimy capsule so they may need to be spun longer. Can be difficult to remove all media, therefore PBS wash is sometimes carried out. |
| growth_condition_temperature | 37.0 |
| growth_condition_time_(h) | No shaking, grow daytime |
| growth_media | THY broth |
| host_location | South Australia, Australia (Adelaide) |
| insert_size_range | 150-2000 |
| isolation_source | Ear |
| key_virulence_genes | Unknown |
| library_construction_protocol | Nextera XT |
| omics | Genomics |
| publication_reference | Infect Immun. 81(2):505-13 (2013) |
| replicate | 1 |
| sample_id | 102.100.100/25788 |
| sample_submission_date | 2016-06-07 |
| sequencer | MiSeq |
| serovar | 3 |
| strain_description | ST180 |
| strain_or_isolate | 180/2 |
| taxon_or_organism | Streptococcus pneumoniae |
| ticket | BRLOPS-847 |
| work_order | Work order 3 |