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Stemcell Metabolomics 102.100.100/29626 LC/MS

Stemcell Metabolomics 102.100.100/29626 LC/MS

Data and Resources

Additional Info

Field Value
Geospatial Coverage
Resource Permissions organization_member
acquisition_mode Scan (85-1200 m/z; 0.8 spectra/s)
analytical_platform LC/MS
archive_ingestion_date 2017-04-21
bpa_dataset_id 102.100.100/52061
cell_type Embryonic stem cells
contextual_data_submission_date 2016-11-11
data_generated 2017-04-21
data_type mzML and tar.gz files
dataset_url https://downloads-qcif.bioplatforms.com/bpa/stemcell/raw/metabolomic/ma/BPAOPS-126/
date_of_transfer 2017-04-21
description Pera lab LC-MS cell samples-Raw
disease_state
extract_protocol According to MA's instruction
facility MA - Bio21
folder_name 20170420_MA_BPA_SC_20161124_LCMS
growth_protocol
instrument_column_type Agilent 1200 LC system/SeQuant ZIC-p HILIC column (5┬Ám, 150 x 4.6 mm; Millipore)
mass_spectrometer Agilent Q-TOF mass spectrometer 6554
method Binary gradient made up of solvent A, 20mM ammonium carbonate solution made up in water, and solvent B, 100% acetonitrile using flow rate of 0.3mL/minute. Solvent B gradient was reduced from 80% to 50% between 0.5 and 15.5 minutes, then reduced to 30% between 15.5 and 17.5 minutes. This gradient was further reduced to 5% between 17.5 and 18.5 minutes, kept at 5% until 23 minutes, increased back up to 80% after 23 minutes and kept at 80% until 29 minutes.
omics Metabolomics
organism_part
plate_number
replicate_group_id
research_group Martin Pera
sample_description Cell extracts
sample_fractionation_extraction_solvent Polar metabolite / Methanol:Water
sample_id 102.100.100/29626
sample_name
sample_submission_date 2016-11-11
species Homo sapiens
stem_cell_line PSC - Pluripotent stages
stem_cell_state Pluripotent stem cell state
submitter Kevin Lau
ticket BPAOPS-126
tissue Cell line
total_samples
well_number