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Stemcell Metabolomics 102.100.100/29606 LC/MS

Stemcell Metabolomics 102.100.100/29606 LC/MS

Data and Resources

Additional Info

Field Value
Geospatial Coverage
Resource Permissions organization_member
acquisition_mode Scan (85-1200 m/z; 0.8 spectra/s)
analytical_platform LC/MS
archive_ingestion_date 2017-04-21
bpa_dataset_id 102.100.100/52064
cell_type ESC
contextual_data_submission_date 2016-01-11
data_generated 2017-04-21
data_type mzML and tar.gz files
dataset_url https://downloads-qcif.bioplatforms.com/bpa/stemcell/raw/metabolomic/ma/BPAOPS-130/
date_of_transfer 2017-04-21
description David Elliot lab LC-MS cell samples-Raw
disease_state BMPR2 mutant
extract_protocol
facility MA - Bio21
folder_name 20170421_MA_BPA_SC_20161115_LCMS
growth_protocol EGM-2V (Lonza CC3202)
instrument_column_type Agilent 1200 LC system/SeQuant ZIC-p HILIC column (5┬Ám, 150 x 4.6 mm; Millipore)
mass_spectrometer Agilent Q-TOF mass spectrometer 6545
method Binary gradient made up of solvent A, 20mM ammonium carbonate solution made up in water, and solvent B, 100% acetonitrile using flow rate of 0.3mL/minute. Solvent B gradient was reduced from 80% to 50% between 0.5 and 15.5 minutes, then reduced to 30% between 15.5 and 17.5 minutes. This gradient was further reduced to 5% between 17.5 and 18.5 minutes, kept at 5% until 23 minutes, increased back up to 80% after 23 minutes and kept at 80% until 29 minutes.
omics Metabolomics
organism_part
plate_number
replicate_group_id 6.0
research_group David Elliot
sample_description p1 Endothelial cells BMPR2 complete null
sample_fractionation_extraction_solvent Polar metabolite / Methanol:Water:Chloroform
sample_id 102.100.100/29606
sample_name
sample_submission_date 2016-01-11
species Homo sapiens
stem_cell_line PSC - Mesendoderm (cardiac differentation)
stem_cell_state Directed differentation
submitter Elizabeth Ling
ticket BPAOPS-130
tissue
total_samples
well_number