ARP Proteomics SwathMS 51637

ARP Proteomics SwathMS Raw Data: Klebsiella pneumoniae KPC2 Pooled sera Replicate 3

Data and Resources

Additional Info

Field Value
Geospatial Coverage
Resource Permissions organization_member
Sequence Data Type proteomics
analytical_platform SWATH-MS
archive_ingestion_date 2019-01-30
contact_researcher Dick Strugnell
contextual_data_submission_date 2018-08-17
culture_collection_date ethics embargo
data_generated True
data_type Main dataset
facility APAF
gradient_time_per_acn 600nL/min, 2% to 10% B, 10 min, 10% B to 35% B, 78 min
gram_stain NEG
growth_condition_growth_phase N/A
growth_condition_media Pooled sera
growth_condition_od600_reading N/A
growth_condition_temperature 37.0
growth_condition_time_(h) 2 hrs exposure to sera
growth_media Pooled sera
host_age ethics embargo
host_disease_outcome ethics embargo
host_dob ethics embargo
host_location ethics embargo
host_sex ethics embargo
isolation_source ethics embargo
key_virulence_genes ethics embargo
lc_column_type Eksigent Ultra nanoLC system (Eksigent), Halo C18, 100 mm × 150 µm, 160Å, 2.7 µm
mass_spectrometer 6600 Triple TOF
omics Proteomics
peptide_resuspension_protocol Tip-probe sonicate 2x 30s in solvent intended for injection (e.g 90% MeCN/0.1% TFA)
replicate 3
sample_id 102.100.100/51637
sample_submission_date 2018-08-21
sample_type HLB-cleaned peptides
serovar ethics embargo
strain_description ethics embargo
strain_or_isolate KPC2
taxon_or_organism Klebsiella pneumoniae
ticket BPAOPS-778
treatment 10mM DTT, 20mM IAA, 1:25µg trypsin 37C 24h