ARP Proteomics SwathMS 51015

ARP Proteomics SwathMS Raw Data: Escherichia coli MS14386 Pooled sera Replicate 1

Data and Resources

Additional Info

Field Value
Geospatial Coverage
Resource Permissions organization_member
Sequence Data Type proteomics
analytical_platform SWATH-MS
archive_ingestion_date 2018-07-18
contact_researcher Steven Djordjevic
contextual_data_submission_date 2017-11-14
culture_collection_date
culture_collection_id E. coli strain 2013-13
data_generated True
data_type Main dataset
facility APAF
gradient_time_per_acn 600nL/min, 2% to 10% B, 10 min, 10% B to 35% B, 78 min
gram_stain NEG
growth_condition_growth_phase N/A
growth_condition_media Pooled sera
growth_condition_od600_reading N/A
growth_condition_temperature 37.0
growth_condition_time_(h) 2 hrs exposure to sera
growth_media Pooled sera
host_age
host_description
host_disease_outcome
host_dob
host_location New South Wales, Australia (Sydney Adventist Hospital)
host_sex
isolation_source Blood
key_virulence_genes Carries a copper resistance operon and hlyE. Putative resistance to five antibiotic classes. Phylogroup B2
lc_column_type Eksigent Ultra nanoLC system (Eksigent), Halo C18, 100 mm × 150 µm, 160Å, 2.7 µm
mass_spectrometer 6600 Triple TOF
omics Proteomics
peptide_resuspension_protocol Tip-probe sonicate 2x 30s in solvent intended for injection (e.g 90% MeCN/0.1% TFA)
protein_yield_facility_ug
protein_yield_total_ug
publication_reference
replicate 1
sample_id 102.100.100/51015
sample_submission_date 2017-11-13
sample_type HLB-cleaned dried peptides
serovar
strain_description ST224
strain_or_isolate MS14386
taxon_or_organism Escherichia coli
ticket BPAOPS-644
treatment 10mM DTT, 20mM IAA, 1:25µg trypsin 37C 24h
work_order