ARP Proteomics SwathMS 50127

ARP Proteomics SwathMS Raw Data: Streptococcus pneumoniae 4496 Pooled sera Replicate 3

Data and Resources

Additional Info

Field Value
Geospatial Coverage
Resource Permissions organization_member
Sequence Data Type proteomics
analytical_platform SWATH-MS
archive_ingestion_date 2018-07-12
contact_researcher James Paton/Richard Harvey
contextual_data_submission_date 2017-10-31
culture_collection_date 1988-06-07
culture_collection_id
data_generated True
data_type Main dataset
facility APAF
gradient_time_per_acn 600nL/min, 2% to 10% B, 10 min, 10% B to 35% B, 78 min
gram_stain POS
growth_condition_growth_phase N/A
growth_condition_media Pooled sera
growth_condition_od600_reading 0.5
growth_condition_temperature 37.0
growth_condition_time_(h) 2 hrs exposure to sera
growth_media Pooled sera
host_age
host_description
host_disease_outcome
host_dob
host_location Victoria, Australia (Melbourne)
host_sex M
isolation_source Blood
key_virulence_genes Low hemolytic Ply
lc_column_type Eksigent Ultra nanoLC system (Eksigent), Halo C18, 100 mm × 150 µm, 160Å, 2.7 µm
mass_spectrometer 6600 Triple TOF
omics Proteomics
peptide_resuspension_protocol
protein_yield_facility_ug
protein_yield_total_ug
publication_reference Infect. Immun. 79(1):4122-30 (2011)
replicate 3
sample_id 102.100.100/50127
sample_submission_date 2017-10-31
sample_type Dried peptides
serovar 1
strain_description Highly virulent in animal models (ST3018)
strain_or_isolate 4496
taxon_or_organism Streptococcus pneumoniae
ticket BPAOPS-627
treatment 25U mutanolysin 37C 2h, 10mM DTT, 20mM IAA, 1:25 ug trypsin
work_order