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Sepsis Genomics Miseq 25788

ARP Genomics Miseq Raw Data: Streptococcus pneumoniae 180/2 THY broth Replicate 1

Data and Resources

Additional Info

Field Value
Geospatial Coverage
Resource Permissions organization_member
Sequence Data Type illumina-shortread
analysis_software_version 2.6.2.3
analytical_facility Ramaciotti
archive_ingestion_date 2016-08-18
contact_researcher James Paton/Claudia Trappetti
contextual_data_submission_date 2016-04-26
culture_collection_date 1989-07-28
culture_collection_id
data_generated True
data_type Main dataset
experimental_replicate 1
experimental_sample_preparation_method Qiagen kit with 100/G genomic tip
facility RAMACIOTTI
gram_stain POS
growth_condition_media THY broth
growth_condition_notes THY broth (recommend using filtered not autoclaved broth as some of the strains don't seem to grow when media has been overheated). If pelleting, serotype 3 forms large loose pellets compared to other pneumococci due to slimy capsule so they may need to be spun longer. Can be difficult to remove all media, therefore PBS wash is sometimes carried out.
growth_condition_temperature 37.0
growth_condition_time_(h) No shaking, grow daytime
growth_media THY broth
host_age
host_description
host_disease_outcome
host_dob
host_location South Australia, Australia (Adelaide)
host_sex
insert_size_range 150-2000
isolation_source Ear
key_virulence_genes Unknown
library_construction_protocol Nextera XT
omics Genomics
publication_reference Infect Immun. 81(2):505-13 (2013)
replicate 1
sample_id 102.100.100/25788
sample_submission_date 2016-06-07
sequencer MiSeq
serovar 3
strain_description ST180
strain_or_isolate 180/2
taxon_or_organism Streptococcus pneumoniae
ticket BRLOPS-847
work_order Work order 3